We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.
Keywords:
Buffers
,Chromatography, High Pressure Liquid
,DNA
,DNA-Directed RNA Polymerases
,Electrophoresis, Polyacrylamide Gel
,Fluorescence Resonance Energy Transfer
,Fluorescent Dyes
,Lasers
,Proteins
,Spectrophotometry, Ultraviolet
,Staining and Labeling
,Transcription, Genetic
