New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB.

Lagrange T, Kapanidis AN, Tang H, Reinberg D, Ebright RH

A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves alpha-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth core promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.

Keywords:

Amino Acid Sequence

,

DNA-Binding Proteins

,

Helix-Turn-Helix Motifs

,

Humans

,

Molecular Sequence Data

,

Nucleic Acid Conformation

,

Promoter Regions, Genetic

,

RNA Polymerase II

,

Transcription Factor TFIIB

,

Transcription Factors

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Transcription, Genetic